Sticky ends from different BsoBI sites may not be compatible. Sticky ends from different AvaI sites may not be compatible. SwaI is typically used at 25☌, but is 50% active at 37☌. Sticky ends from different PflMI sites may not be compatible.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. Software you need to download to your computer prior to lab: Snapgene. Sticky ends from different EcoO109I sites may not be compatible.Ĭ C A N N N N N T G G G G T N N N N N A C C viewing program, SnapGene viewer, prior to arriving to. Show or Hide the SnapGene Zoom Controls Zoom In Pan Across a Sequence History. MN, NLB, SI, LP, AS, JB, VG, TC, and MP conceived of or designed the study. Sticky ends from different BciVI sites may not be compatible. SnapGene Viewer was used to review the chromatograms, and Clustal Omega to compare the DNA and translated RNA sequences.
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